Random by amplified polymorphic DNA analysis of Sylvatic trypanosoma cruzi isolates infers from French Guiana accurate phylogeny.
نویسندگان
چکیده
منابع مشابه
Predominance of Trypanosoma cruzi lineage I in Mexico.
Randomly amplified polymorphic DNA (RAPD) has emerged as an effective genetic marker for analysis of Trypanosoma cruzi population variability. This method has been used to study the genetic variability of Mexican T. cruzi stocks and to relate these results to previous classifications. High clonal diversity was observed among the Mexican populations: 24 RAPD types were scored among 56 stocks ana...
متن کاملRelationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains.
Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to char...
متن کاملMolecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model.
INTRODUCTION For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the mo...
متن کاملGenetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA.
American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed sp...
متن کاملPredominant clonal evolution leads to a close parity between gene expression profiles and subspecific phylogeny in Trypanosoma cruzi.
We investigated the relationships between overall phylogenetic diversity in Trypanosoma cruzi evidenced by multilocus markers (MLEE and RAPD) on the one hand, and gene expression patterns, revealed by mRNA analysis on the other hand. Nineteen laboratory-cloned stocks representative of this parasite's overall phylogenetic diversity and ecogeographical range were analyzed using random amplified d...
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 93 4 شماره
صفحات -
تاریخ انتشار 1998